Public Guest talk: Prof. Dr. Michael Hensel, Division Microbiology, University Osnabrück
The RTG welcomes Michael Hensel as a Guest speaker on Tuesday, 30th of January 2018.
Titel: “Manipulation of the host cell endosomal system by intracellular Salmonella enterica“
Salmonella enterica is an invasive, facultative intracellular pathogen. During intracellular life, the bacteria persist and proliferate in a unique membrane-bound compartment, termed Salmonella-containing vacuole (SCV). The translocation of effector proteins by the Salmonella Pathogenicity Island 2-encoded type III secretion system (SPI2-TSSS) is essential for the intracellular lifestyle and formation of the SCV. The function of SPI2-T3SS effectors also mediate the formation of various tubular vesicular compartments, collectively called Salmonella-induced tubules (SIT). We investigated the physiological relevance of SIT formation for intracellular Salmonella and applied a set of complimentary approaches, i.e. reporter assays, pulse/chase studies with fluid tracers, and fluorescence recovery after photobleaching in cells infected with SPI2-T3SS proficient or deficient Salmonella. Further ultrastructural analyses revealed the membrane organization of SIT and SCV. Collectively, our data demonstrate that the SPI2-T3SS dependent manipulation of the host cell endosomal systems results in formation of a complex, interconnected compartment that provides continuous nutritional supply of Salmonella within the SCV. This compartment may also protect intracellular Salmonella against antimicrobial host activities by continuous dilution of material delivered to the SCV. The molecular mechanisms possibly involved in this pathogenic manipulation will be discussed.
Official announcement: RTG1962_Michael Hensel
Research profil of Prof. Dr. Michael Hensel: Division Microbiology, University Osnabrück [link]
Venue: Department Biology, Building B1, Floor 00, Seminar Room Cell Biology (00.581), Staudtstraße 5, 91058 Erlangen.
Time: 5.00 p.m. (s.t.) –> Drinks will be served, starting at 4.30 p.m.