Public Guest talk: Dr. Satyan Sharma, MPI for Biophysical Chemistry Göttingen

The RTG welcomes Dr. Satyan Sharma as a Guest speaker on Tuesday, 5th of December 2017.

Titel: “Insights into the SNARE fusion machinery using coarse-grained molecular dynamics“

SNARE proteins consisting of vesicular SNARE component synaptobrevin 2 (syb2), and plasma membrane t-SNARE components (syntaxin-1A (stx-1A) and SNAP-25) form a coiled-coil complex. This complex is considered to be the minimal membrane fusion machinery required for the synaptic vesicle exocytosis. A clear picture of the fusion at the molecular level is still unresolved. The coarse-grained molecular dynamics (CGMD) simulations on the fusion of a nanodisc to a multicomponent asymmetric planar bilayer reveal interesting insights into the role of the transmembrane membrane domains (TMDs) of SNARE proteins in fusion. The simulations revealed that zipping of SNARE complexes pulls the syb2 C-terminus deeper into the membrane inducing local curvature. The TMD polar residues Y113, S115 and T116 of syb2 and S281, T282 and G288 of stx-1A form a hydrophilic core between the two distal leaflets facilitating lipid mixing and lead to fusion pore formation. Further, CGMD simulations on t-SNARE clustering shows that t-SNARE form clusters that co-localize with lipid nano-domains in asymmetric bilayers and induce membrane curvature to promote synaptic fusion.

Official announcement: RTG1962_Satyan Sharma

Research profil of Dr. Satyan Sharma: Laboratory for Nanoscale Cell Biology, MPI for Biophysical Chemistry, Göttingen [link]

Venue: Department Biology, Building B1, Floor 00, Seminar Room Cell Biology (00.581), Staudtstraße 5, 91058 Erlangen.

Time: 5.00 p.m. (s.t.) –> Drinks will be served, starting at 4.30 p.m.